Lub fluorescence microscope yog ib hom ntawm optical microscope uas siv lub teeb pom kev zoo los ua kom pom cov qauv thiab excite fluorochromes hauv cov qauv. Qhov teeb pom kev zoo ntawm cov qauv no feem ntau ua nrog lub teeb ci uas emits ultraviolet lub teeb. Lawv tau siv dav hauv kev ua liaj ua teb, kev kho mob thiab kev lag luam.
Lub fluorescence microscope ua haujlwm li cas?
Lub fluorescence microscope siv lub teeb mercury lossis xenon los tsim lub teeb ultraviolet. Lub teeb los rau hauv lub tshuab tsom iav thiab tsoo lub iav dichroic - daim iav uas qhia txog ib qho ntawm cov wavelengths thiab tso cai rau lwm qhov kev hla mus. Daim iav dichroic qhia txog lub teeb ultraviolet mus rau qhov qauv. Qee cov qauv fluoresce ib txwm nyob hauv lub teeb ultraviolet vim tias lawv muaj cov tshuaj fluorescent xws li chlorophyll. Yog tias cov qauv uas yuav tsum tau saib tsis yog ib txwm muaj fluoresce, nws tuaj yeem stained nrog fluorescent dyes hu ua fluorochromes.
Lub fluorescence microscope muaj dab tsi?
1. Lub teeb qhov chaw
Cov teeb pom kev zoo tshaj plaws yog mercury, xenon, thiab LEDs. Mercury muab qhov zoo tshaj plaws ntawm lub teeb rau fluorescence microscope. LEDs tau dhau los ua neeg nyiam vim tias lawv tsis tshua kim dua li lwm qhov chaw thiab lawv siv zog tsawg.
| Mercury Teeb | Xenon Teeb | LED | |
| Wavelength ntawm lub teeb Emitted | 350-370 nm | 400nm ~ 450nm | 400-700 nm |
| Lub neej (teev) | 200-300 | 400-600 | 10000 |
| Qhov zoo | 1. Lub teeb siv hluav taws xob siab, lub teeb pom kev zoo tshaj plaws. 2. Nws emits muaj zog ultraviolet thiab xiav-violet kom excite txhua yam fluorophores. 3. Cov duab ci thiab muaj yeeb yuj. | 1. Lub spectral siv yog ruaj khov. 2. Muaj zog spectral siv nyob rau hauv infrared thiab nruab nrab-infrared. | 1. Yooj yim hloov. 2. Tsis tas yuav ua kom sov. 3. tuaj yeem qhib thiab tawm tam sim. 4. Tswj lub teeb ci siv. |
| Qhov tsis zoo | 1. Lub neej luv luv. 2. Lub sijhawm sov so ntev. | 1. Tshwj xeeb tsis tshua muaj hluav taws xob DC lub hwj chim lub thawv yuav tsum tau ua kom muaj cua sov ntau dhau. 2. Ntau dua li Mercury Teeb | 1. UV teeb tsis muaj zog dua li mercury teeb. 2.Txhua qhov wavelength ntawm lub teeb yuav tsum tau cais LED vim qhov nqaim bandwidths lawv emit. |
Mercury Fluorescent Attachment
LED Fluorescent Attachment
2. Lim lim
Lub tshuab lim excitation yog qhov tseem ceeb rau kev ua haujlwm ntawm fluorescence microscope. Nws hla lub teeb ntawm luv luv wavelength, uas cov xim fluorescent tuaj yeem nqus tau. Tsis tas li ntawd, nws thaiv lwm qhov chaw ntawm lub teeb zoo siab.
Excitation Filter (B, G, U, V)
3. Dichroic Daim iav
Daim iav dichroic yog ib hom optical lim uas qhia txog lub teeb ntawm qee qhov wavelengths thaum kis lwm tus. Nws yog siv nyob rau hauv fluorescence microscopes cais cov excitation thiab emission wavelengths.
4. Emission lim
Lub emission lim kis tsuas yog lub wavelengths emissions los ntawm lub fluorophore thiab thaiv tag nrho cov undesired lub teeb sab nraum lub band - tshwj xeeb tshaj yog cov excitation wavelengths.
5. Fluorescent dyes
Fluorescent dyes yog cov organic tebchaw uas muaj cov khoom ntawm fluorescence, uas lawv tuaj yeem tsim cov duab fluorescent los ntawm emitting qhov sib txawv heev pom lub teeb ntsuab tom qab tau txais kev zoo siab los ntawm lub teeb ci ultraviolet. Feem ntau siv fluorescent dyes yog; DAPI (49,6-diamidino-2-phenylindole), acridine txiv kab ntxwv, auramine-rhodamine, Alexa Fluors, los yog DyLight 488.
Muaj pes tsawg hom fluorescence microscopes?
1. Upright Epifluorescence Microscope:
Nws yog hom tshuaj fluorescence ntau tshaj plaws. Lub excitation ntawm fluorophore thiab nrhiav pom ntawm fluorescence yog ua los ntawm tib lub teeb txoj kev (piv txwv li los ntawm lub hom phiaj). Feem ntau ntawm fluorescence microscopes, tshwj xeeb tshaj yog cov uas siv nyob rau hauv lub neej sciences, yog cov epifluorescence tsim.
BS-2081F Kev Tshawb Fawb Fluorescent Biological Microscope
2. Confocal Fluorescence Microscope:
Confocal Fluorescence Microscope: Hom fluorescence microscope no ua ke nrog laser scanning nrog fluorescent illumination los tsim cov duab. Nws tuaj yeem siv rau ntau yam kev siv, xws li kev kawm cov hlwb thiab cov ntaub so ntswg, kuaj cov protein thiab lwm yam khoom hauv hlwb, thiab ntsuas cov thickness ntawm cov ntaub ntawv.
BS-2081F Kev Tshawb Fawb Fluorescent Biological Microscope
3. Inverted Fluorescence Microscope
Qhov zoo ntawm microscope lub teeb qhov chaw thiab condenser yog nyob rau saum toj, tig downwards. Lub kaum sab xis ntawm illumination yuav tsum nyob ntawm 90 degrees nrog rau qhov chaw ntawm cov qauv kuaj.
BS-2095F Inverted Fluorescent Microscope
Dab tsi yog qhov zoo thiab qhov tsis zoo ntawm fluorescence microscopes?
Qhov zoo:
- Ua kom pom cov hlwb hauv cov kab mob uas muaj sia nyob yam tsis muaj kev puas tsuaj rau lawv
- Muab cov duab daws teeb meem siab nrog cov xim raug
- Tso cai rau kev kawm txog cov txheej txheem nyob hauv cov hlwb
- Siv los txheeb xyuas ntau hom molecules hauv cov hlwb, xws li cov proteins lossis nucleic acids.
Qhov tsis zoo:
-Nws tsuas yog tso cai rau kev soj ntsuam ntawm cov qauv tshwj xeeb hauv ib lub xov tooj ntawm tes nrog cov xim fluorescent.
-Lub photobleaching vim lub electron excitation thaum lub sij hawm tus txheej txheem ntawm fluorescence yuav cuam tshuam rau reactive molecules ntawm fluorescent dyes. Raws li qhov tshwm sim, cov dyes reactive yuav poob lawv cov khoom siv tshuaj fluorescence emission siv.
-Cov hlwb raug cuam tshuam rau cov nyhuv phototoxic tom qab staining nrog fluorescent dyes, raws li cov fluorophore molecules nqus lub zog siab photons los ntawm lub teeb luv-yoj lub teeb.
Dab tsi yog qhov kev siv ntawm fluorescence microscope?
Fluorescence microscopes tau siv dav hauv ntau yam kev tshawb fawb thiab kev siv xws li biochemistry, cell biology, microbiology, immunology, thiab tshuaj.
1. Hauv kev ua liaj ua teb ntawm biology, fluorescence microscope ua kom muaj tseeb thiab cov ncauj lus kom ntxaws qhia txog cellular thiab submicroscopic cellular Cheebtsam thiab kev ua ub no nrog kev pab los ntawm fluorescent dye labeling.
2. Hauv kev kho mob, fluorescence microscope tuaj yeem siv fluorescent reagents los xyuas qhov muaj thiab faib cov kab mob thiab kab mob, los yog pab sau npe cov hom phiaj phais kom yooj yim rau kev phais.
3. Nyob rau hauv kev lag luam mineralogy, fluorescence microscope feem ntau yog siv los kawm cov khoom uas muaj fluorescence yam ntxwv, xws li asphalt, roj av, thee, graphene oxide thiab lwm yam minerals.
4. Hauv cov ntaub ntawv tshawb fawb, fluorescence microscope tuaj yeem siv rau hauv kev lag luam textile lossis kev lag luam ntawv los tshuaj xyuas cov ntaub ntawv fiber ntau.
Post lub sij hawm: Mar-14-2023