Fluorescence microscopy tau hloov pauv peb lub peev xwm los pom thiab kawm cov qauv lom neeg, ua rau peb nkag mus rau hauv lub ntiaj teb sib txawv ntawm cov hlwb thiab cov molecules. Ib qho tseem ceeb ntawm fluorescence microscopy yog lub teeb ci siv los ua kom cov fluorescent molecules nyob hauv cov qauv. Tau ntau xyoo, ntau qhov chaw teeb pom kev zoo tau ua haujlwm, txhua tus muaj nws cov yam ntxwv tshwj xeeb thiab qhov zoo.
1. Mercury Teeb
Lub teeb hluav taws xob mercury siab, xws li 50 txog 200 watts, yog tsim los ntawm quartz iav thiab yog spherical nyob rau hauv cov duab. Nws muaj qee yam mercury hauv. Thaum nws ua haujlwm, qhov paug tawm tshwm sim ntawm ob lub electrodes, ua rau cov mercury evaporate, thiab lub siab sab hauv ntawm lub kheej kheej nce nrawm. Cov txheej txheem no feem ntau yuav siv li 5 mus rau 15 feeb.
Lub emission ntawm lub siab-siab mercury teeb tshwm sim los ntawm disintegration thiab txo cov mercury molecules thaum lub electrode tawm, ua rau lub emission ntawm lub teeb photons.
Nws tso tawm lub teeb muaj zog ultraviolet thiab xiav-violet, ua rau nws haum rau ntau yam khoom siv fluorescent, uas yog vim li cas nws tau siv dav hauv fluorescence microscopy.
2. Xenon Teeb
Lwm qhov uas siv cov teeb pom kev dawb hauv fluorescence microscopy yog lub teeb xenon. xenon teeb, zoo li mercury teeb, muab ib tug dav spectrum ntawm wavelengths ntawm ultraviolet mus rau ze-infrared. Txawm li cas los xij, lawv txawv ntawm lawv qhov excitation spectra.
Mercury teeb tsom lawv cov emission nyob ze-ultraviolet, xiav, thiab ntsuab cheeb tsam, uas ua kom lub cim ntawm ci fluorescent teeb liab tab sis los nrog muaj zog phototoxicity. Yog li ntawd, HBO teeb feem ntau yog tshwj tseg rau cov qauv ruaj khov lossis cov duab tsis muaj zog fluorescence. Nyob rau hauv sib piv, xenon teeb qhov chaw muaj ib tug smoother excitation profile, tso cai rau siv sib piv ntawm txawv wavelengths. Cov yam ntxwv no yog qhov zoo rau kev siv xws li calcium ion concentration ntsuas. Xenon teeb kuj tseem muaj qhov ua kom muaj zog hauv qhov ze-infrared, tshwj xeeb tshaj yog nyob ib ncig ntawm 800-1000 nm.
XBO teeb muaj cov hauv qab no zoo dua HBO teeb:
① Ntau hom spectral siv
② Muaj zog spectral siv nyob rau hauv infrared thiab nruab nrab-infrared cheeb tsam
③ Lub zog tso zis ntau dua, ua rau nws yooj yim dua kom ncav cuag lub hom phiaj lub aperture.
3. LEDs
Nyob rau hauv xyoo tas los no, ib tug tshiab contender tau tshwm sim nyob rau hauv lub realm ntawm fluorescence microscopy lub teeb qhov chaw: LEDs. LEDs muab qhov zoo dua ntawm kev hloov pauv sai hauv milliseconds, txo cov sij hawm raug coj mus kuaj thiab ncua lub neej ntawm cov qauv me me. Tsis tas li ntawd, LED teeb pom kev sai thiab meej lwj, txo qis phototoxicity thaum lub sij hawm ntev nyob ntawm tes.
Piv nrog rau cov teeb pom kev dawb, LEDs feem ntau tawm hauv qhov nqaim excitation spectrum. Txawm li cas los xij, ntau lub LED bands muaj, tso cai rau ntau yam xim fluorescence daim ntaub ntawv, ua LEDs yog ib qho kev xaiv nrov nyob rau hauv niaj hnub fluorescence microscopy setups.
4. Lasers Lub teeb Source
Laser lub teeb qhov chaw yog cov monochromatic thiab kev taw qhia, ua rau lawv zoo tagnrho rau high-resolution microscopy, nrog rau cov kev daws teeb meem loj xws li STED (Stimulated Emission Depletion) thiab PALM (Photoactivated Localization Microscopy). Lub teeb laser feem ntau raug xaiv kom haum rau qhov tshwj xeeb excitation wavelength uas yuav tsum tau rau lub hom phiaj fluorophore, muab siab selectivity thiab precision nyob rau hauv fluorescence excitation.
Kev xaiv ntawm fluorescence microscope lub teeb qhov chaw nyob ntawm qhov kev sim tshwj xeeb thiab cov qauv coj ua. Thov koj xav tiv tauj peb yog tias koj xav tau kev pab
Post lub sij hawm: Sep-13-2023